primary gingival fibroblast; normal, human, adult (ATCC)

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ATCC primary gingival fibroblast; normal, human, adult
Primary Gingival Fibroblast; Normal, Human, Adult, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+gingival+fibroblasts/Primary+Gingival+Fibroblast%3B+Normal%2C+Human%2C+Adult/custom%40pcs-201-018%4041918333
Average 99 stars, based on 517 article reviews

primary gingival fibroblast; normal, human, adult - by Bioz Stars, 2026-10

99/100 stars

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Cell Culture:

Article Title: A non-discoloring bioactive hydrogel for caries management: an in vitro study.
Article Snippet: Collected samples were acid-digested with concentrated nitric acid (Sigma-Aldrich; Merck KGaA, Burlington, MA, USA), centrifuged, filtered, and analysed [20]. .. Cytocompatibility was evaluated using human gingival fibroblasts (HGF-1, ATCC CRL-2014), cultured in Dulbecco's Modified Eagle Medium (Sigma-Aldrich; Merck KGaA, Burlington, MA, USA) supplemented with 10 % fetal bovine serum and 1 % penicillin/streptomycin at 37 ◦C in a 5 % CO2 atmosphere. ..

Article Title: Limosilactobacillus fermentum MG4717 and Limosilactobacillus reuteri MG4722 ameliorate periodontitis in vitro and in a rat model
Article Snippet: The Saos-2 (Korean Cell Line Bank, Seoul, Korea) cells were cultured in minimum essential medium-α (MEM-α; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (P/S; Gibco). .. The human gingival fibroblasts (HGF-1; American Type Culture Collection, Manassas, VA, USA), which are also used as a periodontitis in vitro model, were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco) supplemented with 10% FBS and 1% P/S at 37 ◦C in a 5% CO2 incubator (T.-H. Kim et al., 2024). .. To induce osteoblast differentiation, the medium of Saos-2 was replaced with a MEM-α (Gibco) containing 10% FBS, 1% P/S, 10 mM β-glycerophosphate (β-GP; Sigma-Aldrich, St. Louis, MO, USA), and 10 nM dexamethasone (Sigma-Aldrich) for 10 days (T.-H. Kim et al., 2024).

Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts
Article Snippet: Phospho-p38 MAPK (Thr180/Tyr182; Cat. #9211), phospho-Akt (Ser473; Cat. #9271), and phospho-p42/p44 MAPK (Thr202/Tyr204; Cat. #9101) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Human gingival fibroblasts (HGF-1, ATCC CRL-2014; Manassas, VA, USA) were cultured in Dulbecco's Modified Eagle's Medium (DMEM, ATCC 30–2002) supplemented with 10 % fetal bovine serum (FBS), 100 μg/ml streptomycin, and 100 U/ml penicillin, at 37 °C in a humidified 5 % CO 2 atmosphere. ..

Article Title: Evaluation of different monomer compositions in polymer-infiltrated lithium disilicate (Li 2 Si 2 O 5 ) glass-ceramic
Article Snippet: .. Human gingival fibroblasts (HGF; HGF-1 cell line, CRL-2014, ATCC, Manassas, VA, USA) were cultured in complete medium composed of Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum and 1% penicillin/streptomycin in 5% CO 2 at 37 °C. ..

Modification:

In Vitro:

MTT Assay:

Article Title: Particle Swarm Optimization with Random Forest Surrogates Modelling for Rational Design of Antimicrobial Fluoride Toothpaste Formulations against Clinically Significant Oral Pathogens
Article Snippet: .. We used the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test on human gingival fibroblasts (HGF-1, ATCC CRL-2014) to see how cytotoxic it was. ..

CCK-8 Assay:

Article Title: Development of an Innovative Strontium Gel for Enhanced Antisensitivity, Long-Lasting Film Formation, and Sustained Release of Active Ingredients on Oral Tissues
Article Snippet: .. Sodium hyaluronate (HA, Bloomage Biotechnology, China), sodium alginate (Sinopharm, China), strontium acetate (Sinopharm, China), HAP disc (Sichuan Baiamung Bioactive Materials, China), human gingival fibroblasts (HGF-1, ATCC CRL-2014, Cytokind Biotechnology, China), calcium gluconate (Sinopharm, China), artificial saliva (Phygene, China), and Cell Counting Kit-8 (CCK-8, Phygene, China). ..

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